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mouse bone marrow mesenchymal stem cell lipogenic induced differentiation medium  (Procell Inc)

 
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    Procell Inc mouse bone marrow mesenchymal stem cell lipogenic induced differentiation medium
    Characterisation of <t>mesenchymal</t> stem cells (MSCs). We isolated and cultured MSCs from mouse bone marrow without any treatment and studied their relevant characteristics. (A) Representative photographs of MSCs extracted at different time points during culture at 24 h (a) , 48 h (b) and after passaging (c) ; (B,C) Flow cytometric detection of positive markers CD29, CD44 and Sca-1 and negative markers CD45 and CD11b; Oil red O staining (D) , alizarin red staining (E) and alcian blue staining (F) were used to verify the lipogenic, osteogenic and chondrogenic differentiation ability of MSCs, respectively. scale bar 50 µm in (A,D,E) ; scale bar 100 µm in (F) . MSCs, mesenchymal stem or stromal cells.
    Mouse Bone Marrow Mesenchymal Stem Cell Lipogenic Induced Differentiation Medium, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+bone+marrow+mesenchymal+stem+cell+lipogenic+induced+differentiation+medium/pmc12133893-47-16-26?v=Procell+Inc
    Average 90 stars, based on 1 article reviews
    mouse bone marrow mesenchymal stem cell lipogenic induced differentiation medium - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "IGF-1 secreted by mesenchymal stem cells affects the function of lymphatic endothelial progenitor cells: a potential strategy for the treatment of lymphedema"

    Article Title: IGF-1 secreted by mesenchymal stem cells affects the function of lymphatic endothelial progenitor cells: a potential strategy for the treatment of lymphedema

    Journal: Frontiers in Genetics

    doi: 10.3389/fgene.2025.1584095

    Characterisation of mesenchymal stem cells (MSCs). We isolated and cultured MSCs from mouse bone marrow without any treatment and studied their relevant characteristics. (A) Representative photographs of MSCs extracted at different time points during culture at 24 h (a) , 48 h (b) and after passaging (c) ; (B,C) Flow cytometric detection of positive markers CD29, CD44 and Sca-1 and negative markers CD45 and CD11b; Oil red O staining (D) , alizarin red staining (E) and alcian blue staining (F) were used to verify the lipogenic, osteogenic and chondrogenic differentiation ability of MSCs, respectively. scale bar 50 µm in (A,D,E) ; scale bar 100 µm in (F) . MSCs, mesenchymal stem or stromal cells.
    Figure Legend Snippet: Characterisation of mesenchymal stem cells (MSCs). We isolated and cultured MSCs from mouse bone marrow without any treatment and studied their relevant characteristics. (A) Representative photographs of MSCs extracted at different time points during culture at 24 h (a) , 48 h (b) and after passaging (c) ; (B,C) Flow cytometric detection of positive markers CD29, CD44 and Sca-1 and negative markers CD45 and CD11b; Oil red O staining (D) , alizarin red staining (E) and alcian blue staining (F) were used to verify the lipogenic, osteogenic and chondrogenic differentiation ability of MSCs, respectively. scale bar 50 µm in (A,D,E) ; scale bar 100 µm in (F) . MSCs, mesenchymal stem or stromal cells.

    Techniques Used: Isolation, Cell Culture, Passaging, Staining

    Insulin-like growth factor 1 (IGF-1) secreted by MSC promotes LEPC proliferation and inhibits apoptosis. (A) LEPCs were cultured alone or co-cultured with MSCs for 0 h, 24 h, 48 h or 72 h. Absorbance of LEPCs was measured by CCK-8. **p < 0.01,***p < 0.001 versus LEPCs group; (B,C) LEPCs were co-cultured with MSCs for 72 h. Representative images of EdU + LEPCs and statistical analysis. ***p < 0.001 versus LEPCs group; (D) ELISA for IGF-1 in the culture medium of MSCs or LEPCs. ***p < 0.001 versus MSCs group; (E) Absorbance of LEPCs was detected by CCK-8 after LEPCs were cultured alone, co-cultured with MSCs, or anti-IGF-1 was added to the co-culture system. *p < 0.01 versus LEPCs group; #p < 0.01 versus MSCs + LEPCs group; Cells were then stained with EdU and statistically analysed for positive cells (F,G) . ***p < 0.001 versus LEPCs group; ###p < 0.001 versus MSCs + LEPCs group; (H) CCK-8 experiments to validate the concentration and time dependence of LEPCs on IGF-1. **p < 0.01, ***p < 0.001 versus Control group; (I,J) Immunoblotting of apoptosis-associated proteins and quantification of blotting intensity after 72 h of IGF-1 (100 ng/mL) treatment of LEPCs, GAPDH was used as a loading control. ***p < 0.001 versus LEPCs group; (K) Representative images of apoptotic cells detected by flow cytometry; (L) Quantification of early, late and total apoptotic percentages in untreated or IGF-1-treated LEPCs. *p < 0.05 versus untreated control cells. scale bar 100 µm in B,F; MSCs, mesenchymal stem cells; LEPCs, lymphatic endothelial progenitor cells; IGF-1,insulin-like growth factor 1.
    Figure Legend Snippet: Insulin-like growth factor 1 (IGF-1) secreted by MSC promotes LEPC proliferation and inhibits apoptosis. (A) LEPCs were cultured alone or co-cultured with MSCs for 0 h, 24 h, 48 h or 72 h. Absorbance of LEPCs was measured by CCK-8. **p < 0.01,***p < 0.001 versus LEPCs group; (B,C) LEPCs were co-cultured with MSCs for 72 h. Representative images of EdU + LEPCs and statistical analysis. ***p < 0.001 versus LEPCs group; (D) ELISA for IGF-1 in the culture medium of MSCs or LEPCs. ***p < 0.001 versus MSCs group; (E) Absorbance of LEPCs was detected by CCK-8 after LEPCs were cultured alone, co-cultured with MSCs, or anti-IGF-1 was added to the co-culture system. *p < 0.01 versus LEPCs group; #p < 0.01 versus MSCs + LEPCs group; Cells were then stained with EdU and statistically analysed for positive cells (F,G) . ***p < 0.001 versus LEPCs group; ###p < 0.001 versus MSCs + LEPCs group; (H) CCK-8 experiments to validate the concentration and time dependence of LEPCs on IGF-1. **p < 0.01, ***p < 0.001 versus Control group; (I,J) Immunoblotting of apoptosis-associated proteins and quantification of blotting intensity after 72 h of IGF-1 (100 ng/mL) treatment of LEPCs, GAPDH was used as a loading control. ***p < 0.001 versus LEPCs group; (K) Representative images of apoptotic cells detected by flow cytometry; (L) Quantification of early, late and total apoptotic percentages in untreated or IGF-1-treated LEPCs. *p < 0.05 versus untreated control cells. scale bar 100 µm in B,F; MSCs, mesenchymal stem cells; LEPCs, lymphatic endothelial progenitor cells; IGF-1,insulin-like growth factor 1.

    Techniques Used: Cell Culture, CCK-8 Assay, Enzyme-linked Immunosorbent Assay, Co-Culture Assay, Staining, Concentration Assay, Control, Western Blot, Flow Cytometry

    Combined transplantation of MSCs and LEPCs attenuates hindlimb lymphedema in mice. (A) Images of mouse hind limbs 1 day after surgery; (B) footpad thickness of surgical limbs versus normal limbs in mice,****p < 0.0001; (C) Images of hindlimb oedema in mice at 0, 6 and 12 days after cell transplantation, each set of images from the same mouse; (D) Measurement of footpad thickness in each group of mice after cell transplantation,***p < 0.001 versus other groups; ###p < 0.001 versus LEPCs group; (E) Immunohistochemistry using the lymphatic endothelial marker LYVE-1 in mouse hindlimb samples 12 days after cell transplantation; lymphatic vessels are brown (arrowheads); scale bar 50 µm in a-d,scale bar 20 µm in e-h; (F,G) Analysis of the number of lymphatic vessels and the total area of lymphatic vessels in the immunohistochemical results. *p < 0.05, **p < 0.01 versus PBS group; #p < 0.05 versus LEPCs group. MSCs, mesenchymal stem or stromal cells; LEPCs, lymphatic endothelial progenitor cells; LYVE-1,lymphatic vessel endothelial hyaluronan receptor-1; HPF:high-power field.
    Figure Legend Snippet: Combined transplantation of MSCs and LEPCs attenuates hindlimb lymphedema in mice. (A) Images of mouse hind limbs 1 day after surgery; (B) footpad thickness of surgical limbs versus normal limbs in mice,****p < 0.0001; (C) Images of hindlimb oedema in mice at 0, 6 and 12 days after cell transplantation, each set of images from the same mouse; (D) Measurement of footpad thickness in each group of mice after cell transplantation,***p < 0.001 versus other groups; ###p < 0.001 versus LEPCs group; (E) Immunohistochemistry using the lymphatic endothelial marker LYVE-1 in mouse hindlimb samples 12 days after cell transplantation; lymphatic vessels are brown (arrowheads); scale bar 50 µm in a-d,scale bar 20 µm in e-h; (F,G) Analysis of the number of lymphatic vessels and the total area of lymphatic vessels in the immunohistochemical results. *p < 0.05, **p < 0.01 versus PBS group; #p < 0.05 versus LEPCs group. MSCs, mesenchymal stem or stromal cells; LEPCs, lymphatic endothelial progenitor cells; LYVE-1,lymphatic vessel endothelial hyaluronan receptor-1; HPF:high-power field.

    Techniques Used: Transplantation Assay, Immunohistochemistry, Marker, Immunohistochemical staining



    Similar Products

    mouse bone marrow mesenchymal stem cell lipogenic induced differentiation medium  (Procell Inc)
    90
    Procell Inc mouse bone marrow mesenchymal stem cell lipogenic induced differentiation medium
    Characterisation of <t>mesenchymal</t> stem cells (MSCs). We isolated and cultured MSCs from mouse bone marrow without any treatment and studied their relevant characteristics. (A) Representative photographs of MSCs extracted at different time points during culture at 24 h (a) , 48 h (b) and after passaging (c) ; (B,C) Flow cytometric detection of positive markers CD29, CD44 and Sca-1 and negative markers CD45 and CD11b; Oil red O staining (D) , alizarin red staining (E) and alcian blue staining (F) were used to verify the lipogenic, osteogenic and chondrogenic differentiation ability of MSCs, respectively. scale bar 50 µm in (A,D,E) ; scale bar 100 µm in (F) . MSCs, mesenchymal stem or stromal cells.
    Mouse Bone Marrow Mesenchymal Stem Cell Lipogenic Induced Differentiation Medium, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+bone+marrow+mesenchymal+stem+cell+lipogenic+induced+differentiation+medium/pmc12133893-47-16-26?v=Procell+Inc
    Average 90 stars, based on 1 article reviews
    mouse bone marrow mesenchymal stem cell lipogenic induced differentiation medium - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

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    Characterisation of mesenchymal stem cells (MSCs). We isolated and cultured MSCs from mouse bone marrow without any treatment and studied their relevant characteristics. (A) Representative photographs of MSCs extracted at different time points during culture at 24 h (a) , 48 h (b) and after passaging (c) ; (B,C) Flow cytometric detection of positive markers CD29, CD44 and Sca-1 and negative markers CD45 and CD11b; Oil red O staining (D) , alizarin red staining (E) and alcian blue staining (F) were used to verify the lipogenic, osteogenic and chondrogenic differentiation ability of MSCs, respectively. scale bar 50 µm in (A,D,E) ; scale bar 100 µm in (F) . MSCs, mesenchymal stem or stromal cells.

    Journal: Frontiers in Genetics

    Article Title: IGF-1 secreted by mesenchymal stem cells affects the function of lymphatic endothelial progenitor cells: a potential strategy for the treatment of lymphedema

    doi: 10.3389/fgene.2025.1584095

    Figure Lengend Snippet: Characterisation of mesenchymal stem cells (MSCs). We isolated and cultured MSCs from mouse bone marrow without any treatment and studied their relevant characteristics. (A) Representative photographs of MSCs extracted at different time points during culture at 24 h (a) , 48 h (b) and after passaging (c) ; (B,C) Flow cytometric detection of positive markers CD29, CD44 and Sca-1 and negative markers CD45 and CD11b; Oil red O staining (D) , alizarin red staining (E) and alcian blue staining (F) were used to verify the lipogenic, osteogenic and chondrogenic differentiation ability of MSCs, respectively. scale bar 50 µm in (A,D,E) ; scale bar 100 µm in (F) . MSCs, mesenchymal stem or stromal cells.

    Article Snippet: When the fusion of the cells reached 90%–100%, the cells were cultured for 2–3 weeks using mouse bone marrow mesenchymal stem cell lipogenic induced differentiation medium (Procell, China) or osteogenic induced differentiation complete medium (Procell, China) according to the protocols.

    Techniques: Isolation, Cell Culture, Passaging, Staining

    Insulin-like growth factor 1 (IGF-1) secreted by MSC promotes LEPC proliferation and inhibits apoptosis. (A) LEPCs were cultured alone or co-cultured with MSCs for 0 h, 24 h, 48 h or 72 h. Absorbance of LEPCs was measured by CCK-8. **p < 0.01,***p < 0.001 versus LEPCs group; (B,C) LEPCs were co-cultured with MSCs for 72 h. Representative images of EdU + LEPCs and statistical analysis. ***p < 0.001 versus LEPCs group; (D) ELISA for IGF-1 in the culture medium of MSCs or LEPCs. ***p < 0.001 versus MSCs group; (E) Absorbance of LEPCs was detected by CCK-8 after LEPCs were cultured alone, co-cultured with MSCs, or anti-IGF-1 was added to the co-culture system. *p < 0.01 versus LEPCs group; #p < 0.01 versus MSCs + LEPCs group; Cells were then stained with EdU and statistically analysed for positive cells (F,G) . ***p < 0.001 versus LEPCs group; ###p < 0.001 versus MSCs + LEPCs group; (H) CCK-8 experiments to validate the concentration and time dependence of LEPCs on IGF-1. **p < 0.01, ***p < 0.001 versus Control group; (I,J) Immunoblotting of apoptosis-associated proteins and quantification of blotting intensity after 72 h of IGF-1 (100 ng/mL) treatment of LEPCs, GAPDH was used as a loading control. ***p < 0.001 versus LEPCs group; (K) Representative images of apoptotic cells detected by flow cytometry; (L) Quantification of early, late and total apoptotic percentages in untreated or IGF-1-treated LEPCs. *p < 0.05 versus untreated control cells. scale bar 100 µm in B,F; MSCs, mesenchymal stem cells; LEPCs, lymphatic endothelial progenitor cells; IGF-1,insulin-like growth factor 1.

    Journal: Frontiers in Genetics

    Article Title: IGF-1 secreted by mesenchymal stem cells affects the function of lymphatic endothelial progenitor cells: a potential strategy for the treatment of lymphedema

    doi: 10.3389/fgene.2025.1584095

    Figure Lengend Snippet: Insulin-like growth factor 1 (IGF-1) secreted by MSC promotes LEPC proliferation and inhibits apoptosis. (A) LEPCs were cultured alone or co-cultured with MSCs for 0 h, 24 h, 48 h or 72 h. Absorbance of LEPCs was measured by CCK-8. **p < 0.01,***p < 0.001 versus LEPCs group; (B,C) LEPCs were co-cultured with MSCs for 72 h. Representative images of EdU + LEPCs and statistical analysis. ***p < 0.001 versus LEPCs group; (D) ELISA for IGF-1 in the culture medium of MSCs or LEPCs. ***p < 0.001 versus MSCs group; (E) Absorbance of LEPCs was detected by CCK-8 after LEPCs were cultured alone, co-cultured with MSCs, or anti-IGF-1 was added to the co-culture system. *p < 0.01 versus LEPCs group; #p < 0.01 versus MSCs + LEPCs group; Cells were then stained with EdU and statistically analysed for positive cells (F,G) . ***p < 0.001 versus LEPCs group; ###p < 0.001 versus MSCs + LEPCs group; (H) CCK-8 experiments to validate the concentration and time dependence of LEPCs on IGF-1. **p < 0.01, ***p < 0.001 versus Control group; (I,J) Immunoblotting of apoptosis-associated proteins and quantification of blotting intensity after 72 h of IGF-1 (100 ng/mL) treatment of LEPCs, GAPDH was used as a loading control. ***p < 0.001 versus LEPCs group; (K) Representative images of apoptotic cells detected by flow cytometry; (L) Quantification of early, late and total apoptotic percentages in untreated or IGF-1-treated LEPCs. *p < 0.05 versus untreated control cells. scale bar 100 µm in B,F; MSCs, mesenchymal stem cells; LEPCs, lymphatic endothelial progenitor cells; IGF-1,insulin-like growth factor 1.

    Article Snippet: When the fusion of the cells reached 90%–100%, the cells were cultured for 2–3 weeks using mouse bone marrow mesenchymal stem cell lipogenic induced differentiation medium (Procell, China) or osteogenic induced differentiation complete medium (Procell, China) according to the protocols.

    Techniques: Cell Culture, CCK-8 Assay, Enzyme-linked Immunosorbent Assay, Co-Culture Assay, Staining, Concentration Assay, Control, Western Blot, Flow Cytometry

    Combined transplantation of MSCs and LEPCs attenuates hindlimb lymphedema in mice. (A) Images of mouse hind limbs 1 day after surgery; (B) footpad thickness of surgical limbs versus normal limbs in mice,****p < 0.0001; (C) Images of hindlimb oedema in mice at 0, 6 and 12 days after cell transplantation, each set of images from the same mouse; (D) Measurement of footpad thickness in each group of mice after cell transplantation,***p < 0.001 versus other groups; ###p < 0.001 versus LEPCs group; (E) Immunohistochemistry using the lymphatic endothelial marker LYVE-1 in mouse hindlimb samples 12 days after cell transplantation; lymphatic vessels are brown (arrowheads); scale bar 50 µm in a-d,scale bar 20 µm in e-h; (F,G) Analysis of the number of lymphatic vessels and the total area of lymphatic vessels in the immunohistochemical results. *p < 0.05, **p < 0.01 versus PBS group; #p < 0.05 versus LEPCs group. MSCs, mesenchymal stem or stromal cells; LEPCs, lymphatic endothelial progenitor cells; LYVE-1,lymphatic vessel endothelial hyaluronan receptor-1; HPF:high-power field.

    Journal: Frontiers in Genetics

    Article Title: IGF-1 secreted by mesenchymal stem cells affects the function of lymphatic endothelial progenitor cells: a potential strategy for the treatment of lymphedema

    doi: 10.3389/fgene.2025.1584095

    Figure Lengend Snippet: Combined transplantation of MSCs and LEPCs attenuates hindlimb lymphedema in mice. (A) Images of mouse hind limbs 1 day after surgery; (B) footpad thickness of surgical limbs versus normal limbs in mice,****p < 0.0001; (C) Images of hindlimb oedema in mice at 0, 6 and 12 days after cell transplantation, each set of images from the same mouse; (D) Measurement of footpad thickness in each group of mice after cell transplantation,***p < 0.001 versus other groups; ###p < 0.001 versus LEPCs group; (E) Immunohistochemistry using the lymphatic endothelial marker LYVE-1 in mouse hindlimb samples 12 days after cell transplantation; lymphatic vessels are brown (arrowheads); scale bar 50 µm in a-d,scale bar 20 µm in e-h; (F,G) Analysis of the number of lymphatic vessels and the total area of lymphatic vessels in the immunohistochemical results. *p < 0.05, **p < 0.01 versus PBS group; #p < 0.05 versus LEPCs group. MSCs, mesenchymal stem or stromal cells; LEPCs, lymphatic endothelial progenitor cells; LYVE-1,lymphatic vessel endothelial hyaluronan receptor-1; HPF:high-power field.

    Article Snippet: When the fusion of the cells reached 90%–100%, the cells were cultured for 2–3 weeks using mouse bone marrow mesenchymal stem cell lipogenic induced differentiation medium (Procell, China) or osteogenic induced differentiation complete medium (Procell, China) according to the protocols.

    Techniques: Transplantation Assay, Immunohistochemistry, Marker, Immunohistochemical staining